dat_math [they/them]

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Joined 3 years ago
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Cake day: July 9th, 2021

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  • From the article in Neuroscience about the 12 week experiment (CW clinical gore):

    spoiler

    Long-Evans male (N = 20) and female (N = 20) rats, approximately 6 weeks of age, were obtained from Envigo (Madison, WI, USA).

    After completing all behavioral assessments, rats were anesthetized by exposure to isoflurane and were transcardially perfused at 40 mL/min using a MasterFlex L/S perfusion pump (Cole Parmer; Vernon Hills, IL, USA). During perfusions, approximately 100 mL of phosphate-buffered saline (PBS) solution was pumped through the brain to clear all blood, followed by approximately 200 mL of 4 % paraformaldehyde for tissue fixation purposes. Brains were then extracted and divided sagittally so that the right hemisphere could be processed using the FD Rapid GolgiStain TM Kit (FD NeuroTechnologies, Inc., Columbia, MD, USA), and the left hemisphere could be used for immunohistochemical processing.

    Long Evans rats mean life expectancy is about 3 years. This is one of the tamer experimental protocols, where the animals were not required to undergo terrifically disfigurative surgery to collect data or control conditions in vivo. I have great difficulty imagining how hard it would be to become even slightly attached to those animals and still complete the research duties.















  • There’s an element of truth here, in that parts of the world have a system where farm animals eat stuff humans can’t, such as wild grass, kitchen waste and straw

    The carbon, nitrogen, etc. contained in that grass, waste, and straw should be buried in/returned to the soil to grow plants instead of being farted into our atmosphere. Assuming the land in question is arable in the first place (which I think is valid if it’s producing enough plant matter for grazing to be viable), if managed at all would produce more calories of human-compatible nutrition per calorie invested than harvesting of grazing animals on said land would.